Mito-TEMPO ameliorates hyperthermia-impaired platelet function.

<p>(A) Platelet aggregation was performed as described in Methods. Representative traces from three independent experiments are shown. (B and C) Washed platelets were incubated at the indicated temperatures for 3 h (B), or pre-incubated with Mito-TEMPO, GM6001 and solvent control at 37°C for 15 min, followed by incubation at 42°C for 3 h (C). Western blot was performed as described in Methods. (D and E) Washed platelets were incubated at indicated temperatures for 3 h (D), or pre-incubated without (control) or with Mito-TEMPO and solvent at 37°C for 15 min, and then incubated at 42°C for 3 h (E). Platelet adhesion was performed as described in Methods. The results shown are the mean ± SEM of cell number/mm². *<i>P</i><0.017 (after Bonferroni correction) as compared with RT, **<i>P</i><0.025 (after Bonferroni correction) as compared with control. Mito-TEMPO is labeled as TEMPO.