p62 aggregates sequester LC3-I.

<p>Vectors expressing mRFP-p62, GFP-LC3, or GFP-LC3^A120 were transfected into MEFatg5^−/− cells as indicated (A and B). Images were taken at 48 h time point. The same sets of plasmids were transfected into MEFwt cells as indicated for 72 h. Images were recorded at 20 h and 72 h post transfection (C–F). Scale bar = 25micro. Arrows point to mRFP-p62 only punctae. The inserts were enlarged to show the puncta in detail.