miR-346 promotes osteogenic differentiation of hBMSCs.

<p>(A) qRT-PCR analysis of miR-346 in hBMSCs infected by miR-346-lentivirus or transfected with anti-miR346. The data were normalized to U6. (B) qRT-PCR analysis of the osteoblastic marker genes <i>RUNX2</i>, <i>ALP</i>, and <i>OPN</i> at day 15 of osteoblast differentiation. The data were normalized to <i>β-actin</i>. (C) ALP activity was measured at day 10. (D) ALP staining and Alizarin Red staining were performed at day 10 and day 15, respectively. All data are expressed as mean ± SD from 3 experiments with cells from 3 different donors. *<i>p</i><0.05, **<i>p</i><0.01.