p53 binds to and represses <i>GFI1</i>.

<p>(A) <i>p53</i>^−/− HCT116 cells were transfected with the <i>GFI1</i> promoter (−1933/+468 bp) luciferase reporter construct along with the wild type (WT) or W248 mutant p53. Luciferase activities were measured 36 hrs later and normalized for β-Gal activities. Data are shown as mean ± SD. (B) <i>p53</i>^+/+ and <i>p53</i>^−/− HCT116 cells were transfected with <i>GFI1</i> promoter luciferase reporter construct and treated with Doxo (400 ng/ml) 8 hrs later. Luciferase activities were measured 16 hrs after Doxo treatment. (C) <i>p53</i>^−/− HCT116 cells were transfected with pGL3-basic plasmid containing <i>GFI1</i> promoter fragment (−4840/+184 bp) either alone or together with p53. ChIP assays were carried out using the anti human p53 or an irrelevant species-matched antibody. The indicated regions of <i>GFI1</i> promoter were amplified by PCR. (D) ChIP experiment was carried out on Molt3 cells using the anti human p53 and control antibodies.