HFD after finishing exercise promoted APP CTFβ accumulation in APP-HFD mice.

<p>(<i>A</i>) Immunoblotting analysis of APP full length and APP-CTFβ using APP C-terminus antibody. The number of animals used for immunoblotting analysis is 3–4 per group. Statistical analysis is shown in the right panel. The band of APP CTFβ was normalized by that of APP full length. The band density of the control APP mice was regarded as 100% and that of other groups was relatively indicated. The band density of APP CTFβ in APP-HFD+Ex 0–10 mice tended to be higher compared with that in APP-HFD+Ex 10–20 mice (F _{(4, 13)} = 3.70, p = 0.070). * indicated p<0.05. (<i>B</i>) <i>In vitro</i> enzyme activity assay of neprilysin using fluorescent substrate. The activity of neprilysin in APP-HFD+Ex 0–10 or APP-HFD+Ex 5–15 mice was not different from that in APP-HFD+Ex 10–20 mice (F _{(4, 15)} = 4.10). * indicated p<0.05.