Rarefaction, richness, and diversity analyses of 18S amplicon data.

<p>Seven fungal OTUs were detected through amplicon sequencing. While rarefaction begins to approach saturation, richness estimates predict the presence of at least 11 fungal OTUs indicating that additional sampling may be necessary. This scenario is likely since additional 18S rRNAs from fungal taxa not detected in the 18S amplicons were detected in the shotgun reads (e.g., <i>Fusarium</i> spp.).