Expression of BAX sensitizes DU145 cells to apoptosis.

<p><b>A</b>) Western blot analysis of BAX expression in PC3, C42Luc, and DU145 cells. Cell lysates prepared 6 hours after treatments were probed with antibodies against BAX and β-actin. <b>B</b>) Western blot analysis of cells transfected with green fluorescent protein (GFP) or GFP-BAX chimera. <b>C</b>) Expression of GFP-BAX accelerates apoptosis in DU145 cells. Cells were transfected with GFP or GFP-BAX and apoptosis was analyzed by time-lapse microscopy. At least 100 cells were counted for each treatment. Error bars show standard deviations from the average of four randomly chosen fields.