Substrate specificity analysis of Δ⁹ desaturases (fD9desA, fD9desB, fD9desC, and fD9desD) from <i>Fistulifera</i> sp. on the basis of expression in the yeast <i>Δole1</i> mutant (n = 3).

<p>The yeast strain INVSc-1 transformed with the control vector pYES2.1/V5-His/<i>lacZ</i> (Control vector) served as the positive control. The yeast <i>Δole1</i> mutant transformed with pYES2.1/V5-His/<i>lacZ</i> served as the negative control.</p>a<p>The relative amount of each fatty acid was expressed as a percentage of total fatty acids (± SD) after subtracting the amount of the supplemented fatty acid from the total.