Growth of and EGFP expression from the transformants.

<p>(<b>A</b>) Growth curves of the CC and CG strains in MA2 medium in the presence or absence of uracil. (<b>B</b>) Immunoblotting analysis against total lysates with anti-GFP. The arrowhead indicates the position of EGFP. A CBB-stained part of the gel is shown as a loading control. (<b>C</b>) Fluorescent images showing EGFP signals detected in the CC and CG strains. The CG strains emitted much higher levels of EGFP signals than the CC strains. Exposure times under blue-light excitation are 0.5 sec for 10 D, M4 and CC stains, and 0.2 sec for CG strains. The lower panels show typical G1 and M phase cells (CG1), indicating that the EGFP signals distributed throughout the cytosol. Note that the chloroplasts emitted red autofluorescence. Bars, 5 µm (upper panels) and 1 µm (lower panels). (<b>D</b>) Flow cytometry analysis of EGFP fluorescence. The broken line indicates the mode value calculated from the 10 D data, representing the sum of background and autofluorescence signals.