DAF-16 localization and activity in wild-type and mutant worms.

<p>(A) Transgenic strains expressing DAF-16::GFP under its endogenous promoter. Transgenic controls raised at 20°C are similar to those raised at 20°C then shifted to 35°C for 30 minutes (see panel B for quantification). Close up images of boxed areas are shown. Anterior is to the left in all panels. Low magnification bar, 50 µm; high magnification bar, 25 µm. (B) Quantification of DAF-16::GFP localization in control (n = 157) and <i>vpr-1(tm1411)</i> mutants (n = 49). (−), incubation under normal growth condition; (+), incubation at 35°C for 30 minutes. (C) Magnified images showing transgenic lines expressing GFP under the <i>sod-3</i> promoter. <i>arx-2</i> encodes Arp2. Arrows indicate vulva muscle region. Anterior is to the left in all panels. Bar, 50 µm.