PREBP is critical for the expression of the reporter gene under the <i>pf1-cys-prx</i> promoter.

<p>(A) Schematic diagram of plasmid constructs used for the transient luciferase assay in <i>P. falciparum</i>. The restriction endonuclease recognition sequences shown are E, <i>EcoR</i>I; H, <i>Hind</i>III; K, <i>Kpn</i>I; P, <i>Pst</i>I; S, <i>Sbf</i>I and X, <i>Xho</i>I. (B) The dual luciferase assay. The plasmids shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074701#pone-0074701-g006" target="_blank">Figure 6A</a> were transfected into the parasite synchronized in ring stage. For the dual luciferase assay the pHC1-Rluc plasmid, which expresses Rennila luciferase, was co-transfected in each assay. After 20–24 h, the luciferase activities of the transfected parasites were measured and the ratio of firefly/Rennila luciferase activity was calculated. The data are indicated as means of three independent assays with standard deviation.