Neutrophils are key ifnφ1-producing cells.

<p>(A–C) Effect of myeloid cell depletion (<i>PU.1</i> MO, 3 independent experiments), (D–F) of macrophage depletion (metronidazole treatment of <i>mpeg1:Gal4/UAS:NfsB-mCherry</i> fish, 2 independent experiments), (G–I) of neutrophil-biased depletion (<i>csf3r</i> MO, 2 independent experiments), (J–L) of impairment of emergency granulopoiesis (<i>nos2a</i> MO, 2 independent experiments) and (M–O) of hepatocyte depletion (<i>Tomm22</i> MO, 2 independent experiments) on CHIKV infection. (A, D, G, J and M) Disease score at 3 dpi. (B, E, H, K and N) Survival of infected zebrafish. Data pooled from the independent experiments. (C, F, I, L and O) qRT-PCR of viral <i>E1</i> transcripts at 24 hpi. Mean ± s.e.m of 6 pools of 5 larvae from 2 (C, F, L) or 1 (I, O) independent experiments. (***<i>P</i> &lt; 0.001; **<i>P</i> &lt; 0.01; *<i>P</i> &lt; 0.05; ns - not significant).