CIP restores insulin secretion after inhibition by elevated Cdk5 activity.

<p>Min 6 cells were transiently infected with EV, CIP, p25, p25/CIP, and p25/DNcdk5 constructs respectively. After 48 hours infection, cells were washed and starved for 2 hrs. Then, cells were stimulated by 3 mM and 25 mM glucose respectively for 2 hrs. Insulin secreted into the supernatant was measured using LINCO, ELISA Kit. CIP increased the insulin secretion after inhibition by p25 (compared p25/CIP to p25), (* P<0.01; ** P<0.05). Data represent mean ± SE of three experiments.