Conditional intestinal epithelial HDAC1/2 loss disrupts cell lineage commitment.

<p><b>A</b>. Jejunal tissue sections from four-month-old control (Ctrl, left panel) or conditional intestinal epithelial HDAC1/2 (HDAC1/2ΔIEC, right panel) mice were stained with Best’s Carmine. Arrows indicate stained Paneth cells. Magnification: 20 X. <b>B</b>. Jejunal tissue sections from four-month-old control (Ctrl, left panel) or conditional intestinal epithelial HDAC1/2 (HDAC1/2ΔIEC, right panel) mice were stained with an antibody against lysozyme, a Paneth cell marker. Arrows indicate stained Paneth cells. Magnification: 20 X. C. Total RNAs were isolated from control and HDAC1/2 IEC-specific jejunum (n=4-6). Expression levels of lysozyme and Defa1 (cryptdin), two Paneth cell markers, were determined by qPCR, with Pbgd as a control. Results represent the mean ± SEM (* p≤0.05). <b>D</b>. Total RNAs were isolated from control and HDAC1/2 IEC-specific colons (n=4-5). Expression levels of Cdx2 and Sucrase-isomaltase (Sis), a small intestine enterocyte marker, were determined by qPCR, with Pbgd as a control. Results represent the mean ± SEM (* p≤0.05). <b>E</b>. Colon tissue sections from four-month-old control (Ctrl, left panel) or conditional intestinal epithelial HDAC1/2 (HDAC1/2ΔIEC, right panel) mice were stained with an antibody against Sucrase isomaltase (Sis), a small intestine enterocyte marker. Magnification: top panels: 20 X; bottom panels: 40 X.