Dose dependent reduction in CHIKV copies/ml serum after injection with siRNA Chik-1 and Chik-5 in swiss albino and C57BL/6 mice infected with CHIKV.

<p>Swiss albino and C57BL/6 mice were infected with CHIKV i.v. (1×10⁶ PFU CHKV; 100 µl of 10⁷pfu/ml). After 72 h p.i. swiss albino mice were inoculated i.v. with <b>A</b>) Scrambled siRNA (siRNA against chandipura virus), 250 µg, 500 µg and 1 mg/kg body weight Chik-1 siRNA (n = 3 for each treatment and time point), <b>B</b>) Scrambled siRNA, 250 µg, 500 µg and 1 mg/kg body weight Chik-5 siRNA (n = 3 for each treatment and time point). After 72 h of p.i. C57BL/6 mice were inoculated i.v. with <b>C</b>) Scrambled siRNA, 250 µg, 500 µg and 1 mg/kg body weight Chik-1 siRNA (n = 3 for each treatment and time point), <b>D</b>) Scrambled siRNA, 250 µg, 500 µg and 1 mg/kg body weight Chik-5 siRNA (n = 3 for each treatment and time point). At indicated time after injection of siRNA blood was collected from eye and RNA was isolated from serum. CHIKV E3 RNA copies were quantitated using real time RT-PCR. Values are given as LOG10 RNA copies/ml serum.