Adipogenic potential of BOCs.

<p>Sorted Dmp1Cre/Ai9⁺ and Dmp1Cre/Ai9⁻ cells were cultured under basal conditions for 3 days (indicated as day 0), then treated with adipogenic medium. Unlike Dmp1Cre/Ai9⁺, Dmp1Cre/Ai9⁻ cells showed evidence of adipogenesis (A). Adipogenic differentiation was assessed by Oil Red O staining (B) and adiponectin and adipsin expression levels by real-time PCR analysis (C). Real-time PCR results are presented as mean±SEM of data normalized to Gapdh expression and pooled from two biological replicates. * P<0.05 compared to Dmp1Cre/Ai9⁺ day 9 as determined by two-tailed Student’s t-test. Statistical analysis was only performed where expression was detectable in both samples. ND, not detectable.