MTX induces dose- and time-dependent cell viability lost and activates caspase 3 activities in IEC-6 cells.

<p>IEC-6 cells were treated with (A) different concentration of MTX (0, 0.01, 0.1, 1, 10 and 100 μM) for 24 hrs, or (B) 0, 1, 10, 100 μM of MTX for 24, 48, and 72 hrs, and the cell viabilities were determined by WST-1 assay. (C) The caspase 3 activities were detected in cells treated with indicated concentration of MTX for 24 hrs. Values are means ± S.D. of at least three independent experiments. *, <i>p</i><0.05.