Immune response in mice after transfers of MGL2⁺ dDCs <i>in vivo</i>.

<p>In the following panels, data obtained from untreated mice, mice that received FITC⁺MGL2⁺ cells, and mice that received FITC⁺CD103⁺ cells are indicated as “Ctrl,” “MGL2,” and “CD103,” respectively. (A) FITC-specific ear swelling 1 day after elicitation is shown in µm. (B) Relative quantity of mRNA for <i>Ifng, Il4, Il17a, Il5, Il13</i> and <i>Foxp3</i> was measured by the quantitative real-time PCR in LN cells collected from recipient mice 1 day after elicitation. The values are shown as the mean ± SD from three independent measurements, and the levels of significance for the differences are: *** for p<0.005 and n.s. for not significant. (C) Results of flow cytometry analysis of intracellular cytokines in CD4⁺ T cells from LNs collected from recipient mice 1 day after elicitation are shown. The numbers in the panels indicate the percentages of IFN-γ⁺IL-4^–CD4⁺ T cells, IFN-γ^–IL-4⁺CD4⁺ T cells, IFN-γ⁺IL-17A^–CD4⁺ T cells, and IFN-γ^–IL-17A⁺CD4⁺ T cells in the total CD4⁺ T cells. (D) The percentages of IFN-γ⁺IL-4^–CD4⁺ T cells, IFN-γ^–IL4⁺CD4⁺ cells, and IFN-γ^–IL-17A⁺CD4⁺ T cells in total CD4⁺ T cells are illustrated in bar graphs. Values in the Y-axis indicate the mean ± SD from three separate experiments, * indicates that the difference is statistically significant (p<0.05) and n.s. indicates that the difference is not statistically significant. (E) FITC-specific IgG1 and IgG2a in the sera of mice that received a transfer of FITC⁺MGL2⁺ cells or FITC⁺CD103⁺ cells were determined. The y-axis indicates the relative antibody binding to FITC. Mean ± SD from 9 separate experiments are shown, and *, ***, and n.s. indicate p<0.05, p<0.005, and difference not statistically significant, respectively. (A–E) The experiments were independently performed more than three times.