Paucity in IL-12 production by MGL2⁺ dDCs.

<p>(A) Flow cytometry analysis of intracellular IL-12_p40 in MGL2⁺ dDCs, MGL2^–CD103^– skin-derived DCs, and CD103⁺ dDCs in skin-draining LNs under naïve or sensitized conditions. MGL2⁺ dDCs are shown as “MGL2,” MGL2^–CD103^– DCs are shown as “DN” and CD103⁺ dDCs are shown as “CD103.” Cells from naïve mice are shown as “Naïve,” and cells from sensitized mice are shown as “Sensitized.” Areas shaded in gray indicate the staining pattern with an isotype control antibody. The numbers represent the mean fluorescence intensity (MFI) of each skin-derived DC subset. (B) The MFI of IL-12_p40 for each skin-derived DC subset is shown in panel A in bar graphs. Data are shown as the mean ± SD of three biological replicates (n = 3); n.s. indicates that the difference is not statistically significant. (A–B) The experiments were independently performed three times. (C) Concentrations of IL-12_p40 and IL-12_p70 in culture supernatant of MHCII^highMGL2⁺ dDCs, MHCII^highCD103⁺ dDCs, FITC⁺MGL2⁺ dDCs, and FITC⁺CD103⁺ dDCs were measured by the Bio-Plex Suspension Array System and are shown in the panels as “Naive MGL2,” “Naïve CD103,” “Sensitized MGL2,” and “Sensitized CD103,” respectively. The experiments were independently performed two times.