Cell cycle arrest of LMeC indirect co-cultured with cAT-MSC-IFN-<i>β</i>.

<p>The cell cycle phase distribution of LMeC was analyzed after harvest by flow cytometery. (A, B) cAT-MSC:LMeC 1∶1 and (C, D) 4∶1. The LMeC cells co-cultured with cAT-MSC-IFN-<i>β</i>, showed increase in the G0/G1 phase of the cell cycle compared to the controls (<i>p</i><0.05 and <i>p</i><0.01 at a cAT-MSC-IFN-<i>β</i>/LMeC ratio of 1∶1 and 4∶1 respectively). G1 arrest occurred concurrently with a reduction in the percentage of S phase cells (<i>p</i><0.01 and <i>p</i><0.001 at a cAT-MSC-IFN-<i>β</i>/LMeC ratio of 1∶1 and 4∶1 respectively). The P-value was obtained using one-way ANOVA with <i>post-hoc</i> Bonferroni's multiple comparison by comparing treated cAT-MSCs (cAT-MSC-Mock or cAT-MSC-IFN-<i>β</i>) with control (LMeC alone) for each phase of the cell cycle. Data are representative of three independent experiments with similar results. * <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001.