Effects of IDO deficiency on intrahepatic triglycerides, the serum ALT levels, and oxidative stress in the experimental mice.

<p>(A) Hepatic lipids were extracted from the frozen livers of the experimental mice, and the triglyceride levels were measured. (B) At sacrifice, blood samples were collected and the serum levels of ALT were assayed. (C) The hydroperoxide levels in the serum at the end of the experiment were determined using the d-ROM test. (D) Total RNA was isolated from the livers of the experimental mice, and the expression levels of SOD-1, SOD-2, and GPx mRNA were examined using quantitative real-time RT-PCR with specific primers. The values are expressed as the mean ± SD. * <i>P</i> <0.001, ** <i>P</i> <0.01, *** <i>P</i> <0.05.