Time course for Al(OH)₃ exhaustion of the three complement pathways.

<p>NHS was incubated with 30(OH)₃ at 37°C for the indicated times and MAC deposition by the three pathways (alternative pathway (AP), lectin pathway (LP), classical pathway (CP)) was measured in the supernatant after centrifugation. A. Short term effect of Al(OH)₃ on the three complement pathways. B. Long term effects of Al(OH)₃ on complement activity. The figures show data from 3 independent experiments with means±SD. P-values for the reduction of complement activity were calculated using 2-way ANOVA and Bonferroni posttest (*p<0.05; **p<0.01; ***p<0.001). Positive, negative and blank controls provided by the kit manufacturer were analysed with each experiment.