Deposition of complement occurs on human vaccines containing Al(OH)₃.

<p>A human serum sample was treated with A) Al(OH)₃, B) the Al(OH)₃-containing vaccine diTe booster (diphtheria and tetanus vaccine) or C) the Al(OH)₃-containing Havrix-vaccine. The Al(OH)₃-containing precipitate was isolated by centrifugation and used for coating on a polystyrene surface and analysed for the complement components C3, C4, mannan-binding lectin (MBL), Properdin, factor B and membrane attack complex (MAC) using specific antibodies. The figure shows the mean of double determinations±SD from one of two independent experiments. Statistically significant differences were calculated using 2-way ANOVA and Bonferroni posttest for the specific antibody versus the control (β-gal) (*p<0.05, ***p<0.001).