Time course for complement components deposition on Al(OH)₃.

<p>A) Serum was incubated with 30 mM Al(OH)₃ for the indicated times at 37°C and centrifuged. The protein composition of the precipitate was analysed using ELISA with monoclonal antibodies directed against the indicated complement components. Data are means±SD of 2 independent experiments. Controls were as in fig. 6. B) Time-dependent generation of the membrane attack complex (MAC The results are the means of 3 independent experiments. Statistical analysis was performed using 2-way ANOVA and Bonferroni posttest between the specific antibody versus control (β-gal) (A) or between time points (B) (**p<0.01; ***p<0.001; ns = not significant).