The effect of inhibitors on complement activation by Al(OH)₃.

<p>Membrane attack complex (MAC) deposition was measured using a specific antibody on the precipitate of Al(OH)₃-treated serum (30 mM Al(OH)₃ for 1 hour at room temperature) with or without prior addition of 10 mM EDTA or 10 mM EGTA +10 mM MgCl₂ comparing normal human serum with mannan binding lectin (MBL)-deficient serum, C3-depleted serum and fB-depleted serum. The figure shows means of 3 independent experiments±SDs. 2-way ANOVA and Bonferroni posttest showed a significant difference only for NHS and MBL-deficient serum after addition of EDTA (***p<0,001). Controls included untreated and lipopolysaccharide-treated serum.