DCLK1 negatively regulates <i>miR-143/145</i>.
- Sripathi M. Sureban (455805)
- Randal May (455806)
- Dongfeng Qu (455807)
- Nathaniel Weygant (455808)
- Parthasarathy Chandrakesan (455809)
- Naushad Ali (455810)
- Stan A. Lightfoot (455811)
- Panayotis Pantazis (340343)
- Chinthalapally V. Rao (455812)
- Russell G. Postier (356178)
- Courtney W. Houchen (356179)
Publication date
September 2013
DOIAbstract
<p>Following the knockdown of DCLK1 in AsPC-1 tumor xenografts, a significant upregulation of <i>miR-143/145</i> cluster (A) and <i>miR-145</i> miRNA (B) by real-time RT-PCR. C, A decrease in luciferase activity (luciferase units) following transfection with plasmid-encoding luciferase containing the <i>miR-145</i> binding site was observed following the knockdown of DCLK1 in AsPC-1 human pancreatic cancer cells. Knockdown of DCLK1 also resulted in the downregulation of KRAS (D) and RREB1 (E) mRNA, downstream targets of <i>miR-143/145</i> miRNA cluster, analyzed using real-time RT-PCR. Values are given as average ± SEM, and <i>asterisks</i> denote statistically significant differences (*<i>p</i> < 0.01) compared with Control (NP alone).</p
Add to ORKG