The effects of poly(P) on the viability of macrophages.

<p>The cells were pretreated with Pi, poly(P)₁₄, poly(P)₆₀, or poly(P)₁₃₀ (1 mM) for 5 min and stimulated with LPS (100 ng/mL) for 24 h. Cell viability was then determined using the WST-8 assay (A) and trypan blue exclusion assay (B). H₂O₂ (250 µM, 24 h) was used as a positive control. Values are expressed as means ± SD of the percentage of cell viability (A) or cell number (B) compared with basal from three independent experiments using different cell preparations.