Different expression pattern of pro-inflammatory mediators and receptors in induced and tolerant iDCs at 27h.

<p>The relative gene expression of pro-inflammatory effectors such as CXCL8 (A), CCL1 (B), CXCL10 (C), IL-6 (D), IL-10 (E) and IL-17A (F) shows significant upregulation in induced (N+PGN) as compared to the naïve (N) iDCs. The expression of CXCL8 (A), CCL1 (B), IL-6 (D) and IL-17A (F) shows further upregulation in tolerant iDCs (T+PGN) as compared not only to the naive but to the induced iDCs as well. In contrast, the expression of CXCL10 (C) in tolerant iDCs is significantly downregulated when compared both to naïve and induced iDCs. In contrast to the effector molecules, receptors involved in inflammation such as gp130 (G), IL-10RA (H) and IL-17RA (I), were significantly down regulated in both induced and tolerant iDCs as compared to naïve cells. The ratio of each mRNA relative to the 18S rRNA was calculated using the 2^-ΔΔCT method. Data are representative of 3 or more independent experiments and are presented as interquartile range (box) with median (horizontal black bar) and minimum and maximum values. The significance of differences between sets of data was determined by Student’s paired t-test using SPSS Statistics; *<i>p</i><0.05.