Content and nuclear localization of Dot COM.

<p>(<b>A</b>) Immuno-RNA FISH staining with antisense 508 riboprobe (red) and anti-lamin antibody (green) in follicle cells of <i>ISO1A</i> line. (<b>B</b>) DNA/RNA FISH staining for <i>COM</i> transcripts (green), the <i>COM</i> locus (red) and DNA (blue) in follicle cells in <i>ISO1A</i> (top), <i>w^IR6</i> (middle) and <i>Rev</i> (bottom) lines. (<b>C</b>) Double FISH staining in <i>ISO1A</i> follicle cells with different combinations of the riboprobes indicated. cl11 and cl14 probes recognize respectively cluster 11 (both strands indicated by + and −) and cluster 14 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072752#pone.0072752-Malone1" target="_blank">[5]</a>. In all rows, the merge RGB image is shown alongside with the individual channels in white and green. DNA is indicated in blue.