Evaluation of the optimum concentration of calcimimetics on oAFMSCs treated for 14 days.

<p>(<b>A</b>) MTT assay performed at day 14 on oAFMSCs shows a reduction in cell viability upon increasing the concentrations of R-568 or S-568 (0.1–10 µM) (*p<0.01 vs Basal). (<b>B</b>) Decreasing cell number trend on day 14 at enhanced concentrations of calcimimetics R-568 and S-568 (0.1–10 µM) (*p<0.001 vs Basal). The number of cells is normalized per cm². All histograms represent the mean ± SD of three independent experiments. (<b>C</b>) Bright field images of oAFMSCs at increased concentrations of calcimimetics showing the cell morphology and the accumulations of vacuoles at high concentrations (enlargement in the right corner). Magnification 10x.