NCINI potency is unaffected by LEDGF expression levels during virus production.

<p>(<b>A</b>) Western blot analysis of HEK293T cells overexpressing LEDGF during virus production. (<b>B</b>) Infectivity of virus produced from mock (black bars) and LEDGF overexpressing (light grey bars) HEK293T producer cells. (<b>C</b>) Western-blot analysis of LEDGF expression in HEK293T cells generated from mock transfection or stable transfection with control scrambled or LEDGF-specific shRNAs. (<b>D</b>) Infectivity of virus produced from HEK293T cells that were mock transfected (black bars) or stably transfected with scrambled shRNA (hatched bars) or LEDGF shRNA #8 (white bars) and #3 (grey bars). Infectivity measurements for B and D represent mean ± SD values obtained from triplicate measurements from a representative experiment.