FN 1 was a direct target of miR-206.

<p>(A) Left panel shows predicted duplex combination between human FN 1 3’-UTR and miR-206 (upper, sequence of mouse FN 1 3’-UTR including miR-206 binding site; lower, sequence of miR-206). Right panel shows dual luciferase assay of H441 cells cotransfected with pMIR-FN 1 and miRNA (mock, miR-3965, miR-206 or miR-206 + miR-206AS). (B) Real-time PCR of H441 cells transfected with miR-206 or mock to detect FN 1 expression. (C) Western blotting of H441 cells transfected with miR-206 or mock to detect FN 1 expression. (D) Relative expression of FN 1 for neonatal mice was detected using real-time PCR. The relative expression of FN 1 in BPD mice compared with air-exposed control mice. (E) Detection of FN 1 expression in BPD lung and controls by immunohistochemical staining. FN 1 (red) was expressed at higher levels in BPD samples (P21) compared with air-exposed controls (P21). Bars represent 20 µm. (F) Relative expression in patient samples of FN 1 in BPD by using real-time PCR.