Overexpression of HA-CXCR7 in 143B-<i>LacZ</i> cells diminished primary intratibial tumor growth, but promoted metastasis to the lung and the auriculum cordis.

<p>(A) Representative X-ray images of a tumor cell injected leg (tumor leg) and of the contralateral non-injected leg (control leg) of mice injected with 143B-<i>LacZ</i>-EV (EV) cells or with 143B-<i>LacZ</i>-HA-X7 (HA-X7) cells at day 27 after tumor cell injection and representative HA immunohistochemical staining of HA-CXCR7 on sagittal sections of EV and HA-X7 cell injected tibiae and mean calculated volume of intratibial tumors in EV and HA-X7 cell-injected mice at day 27 after tumor cell injection. (B) Representative lung whole mounts with X-gal-stained metastases (blue) obtained from EV (arrows indicate the metastases) and HA-X7 cell injected mice and mean (± SEM) numbers of metastases on the lung surface of respective animals. (C) Whole mounts of auriculum cordis enlarged from (B) with X-gal stained metastatic lesions and mean (± SEM) percentage of mice in indicated groups with auriculum cordis metastatic lesions. Six and 7 mice in the EV and the HA-X7 groups, respectively, were analyzed. *p≤0.05.