Inhibition of the mitochondrial cytochrome <i>c</i> oxidase activity by Ca²⁺.

<p>Oxidation of 15 µM ferrocytochrome <i>c</i> by mitochondria from different tissues (0.5–1.5 mg protein/ml) was followed spectrophotometrically under low-turnover conditions as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074436#s2" target="_blank">Materials and Methods</a>” and legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074436#pone-0074436-g007" target="_blank"><b>Figure 7</b></a>. The data correspond to inhibition induced by addition of excess Ca²⁺ (200 µM on the backgrpound of 100 µM EGTA). The data are average ± SE for 3–5 measurements.