Effect of MV and LPS on HMGB1 expression and cytokines production.

<p>Quantitative RT-PCR was performed with total RNA from each animal, and HMGB1 mRNA expression in the lung was shown as percentage over β-actin gene in the same sample (A). HMGB1 protein expression was determined by Western blotting in the lung and the intensity ratio of HMGB1 to β-actin for each band is shown (B). ELISA was performed for protein levels of IL-1β (C), IL-6 (D), TNF-α (E), MIP-2 (F) and HMGB1 (G) in BALF from each animal. Data shown are means±SD from 6 rats per group. *, p<0.05 vs sham group; #, p<0.05 vs LPS group.