Analysis of 16S rRNA gene-based real-time PCR amplification of intestinal bacterial groups.

<p>Detected DNA levels in luminal content from (A) ileum, (B) cecum and (C) colon of naive (open circles) and acutely infected (day 14 post infection, black circles) mice were calculated as 16S rRNA copy numbers per ng DNA extract. Mean ± SEM is shown for 4-5 mice per group. DB: domain bacteria, γ-p/EB: γ<i>-Proteobacteria/Enterobacteriaceae</i>, LB: Lactobacilli, C IV: <i>Clostridium</i> cluster IV, C XIV a/b: <i>Clostridium</i> cluster XIV a/b, B: <i>Bacteroides</i> group, MIB: mouse intestinal <i>Bacteroides</i>, EC: Enterococci, n.d.: not detectable. * p < 0.05; ** p < 0.005 compared to naïve.