DCreg-treated BMT mice show reduced histologic evidence of GVHD.

<p>Young and older B6 mice were treated as described in Figure 3. Colon sections from (A) untreated B6 mice, (B) BMT mice, and (C) DCreg-treated BMT mice were stained with H & E. Original magnification was 20X (top panels) and 60X (lower panels). Tissues in (B) and (C) were obtained 1 wk post-BMT. Circles, necrosis/apoptotic bodies/cellular debris. Boxes, crypt degeneration/increased interstitial space. Representative images of 4-8 mice/group. (D) Total small intestine and colon histology scores of BMT mice 1 wk post-BMT, and DCreg-treated BMT mice 1 and 2 wk post-BMT were determined by adding individual organ scores as described in Methods. There were no statistical differences between young and older groups that received the same treatment. Data are mean ± SEM. N = 3-4 mice/group. * = p<0.05, ** = p<0.01, *** = p<0.001.