Rescue of the mild Egl phenotype shown by <i>kcnl-2</i>(<i>tm1885</i>) worms.

<p>A. <i>kcnl-2</i>(tm1885) animals retain a significantly greater number of eggs <i>in utero</i> relative to N2 animals (P<0.0001). The number of eggs <i>in utero</i> in heterozygotes (<i>kcnl-2</i>(-/+)) is significantly greater than that of the N2 animals (p=0.007), but significantly less than the average number of eggs found in <i>kcnl-2</i>(tm1885) animals (p=0.008). B. Transformation of <i>kcnl-2</i>(tm1885) with 1 ng/µl, 10 ng/µl or 90 ng/µl of <i>p</i>_{<i>kcnl-2</i>}<i>kcnl-2</i>(taa2)<i>::gfp</i> gives the transgenic lines KCNL-2(OE-3) (strain VK2220), KCNL-2(OE-4) (strain VK1004) and KCNL-2(OE-5) (strain VK1041), respectively. The average number of eggs retained <i>in utero</i> by KCNL-2(OE-3) was not significantly different from the N2 animals (P=0.8) but was significantly different from the <i>kcnl-2</i>(tm1885) organisms (p<0.0001). KCNL-2(OE-4) and KCNL-2(OE-5) retain fewer eggs <i>in utero</i> than N2 animals (p<0.005; p<0.0001, respectively) and <i>kcnl-2</i>(tm1885) animals (p<0.0001) (Student’s <i>t</i> test). C. Egg-staging assays show that the proportions of young eggs from KCNL-2(OE-2) (54.0±4.3%), KCNL-2(OE-4) (46.0±3.9%), and KCNL-2(OE-5) (46.6±6.7%) are significantly greater than those of N2 (7.2±1.3%) & <i>kcnl-2</i>(tm1885) worms (1.6±1.6%) (p<0.05; n=3, Kruskal–Wallis <i>H</i> test).