The role of the regulation of PKCα by miR-203 in cellular proliferation.

<p>A, validation of the siRNA against PKCα. Three siRNAs targeting different sites of human PKCα cDNA and a scrambled control siRNA were transfected into A549 cells using Lipofectamine 2000. Western blot analysis depicts PKCα protein levels at 24 h post-transfection. Normalized quantification of the immunoblots was carried out from independent experiments. Data are presented as the mean ± S.D. (** <i>p</i><0.01). B, cell viability assay at 12, 24, 36, and 48 h after transfection of A549 cells with equal doses of control siRNA or si-PKCα, or C, equal doses of scrambled ncRNA or pre-miR-203 (mean ± S.D.; ** <i>p</i><0.01). D, Western blot analysis of the protein level of PKCα in A549 cells transfected with ncRNA, pre-miR-203, or pre-miR-203 plus PKCα overexpression plasmid, and a statistical analysis is shown in the right panel (mean ± S.D.; ** <i>p</i><0.01). E, Immunoblot for PKCα protein in A549 cells that were either mock transfected or transfected with PKCα overexpression plasmid. F, cell viability assay at 12, 24, 36, and 48 h after transfection of A549 cells with ncRNA, pre-miR-203, pre-miR-203 plus PKCα overexpression plasmid, or PKCα overexpression plasmid alone (mean ± S.D.; ** <i>p</i><0.01; *** <i>p</i><0.001).