Interactions between poly(A)+ mRNA and cytoskeletal elements in HDF.

<p>(<b>A</b>) β-tubulin, vimentin and phalloidin IF, poly(A)+ mRNA, and PLA between poly(A)+ mRNA and the cytoskeletal elements in HDF were imaged with a laser-scanning confocal microscope. Merged images of the cytoskeleton (white), poly(A)+ mRNA (red), PLA (green) and nuclei (blue) are shown. Single image plane is represented. Inset, images of boxed regions. Scale bar, 10 µm (2 µm in insets). (<b>B</b>) The mean FMTRIP volume was similar (Kruskal-Wallis One Way ANOVA on Ranks, p=0.5) in cells, where the interactions between poly(A)+ mRNA and β-tubulin (n=25, mean=706µm³, s.d.=427µm³), vimentin (n=33, mean=780µm³, s.d.=405µm³), or phalloidin (n=23, mean=916µm³, s.d.=346µm³) were quantified. (<b>C</b>) The mean percentage of FMTRIP colocalized with PLA (PLA-FMTRIP) was significantly different (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074598#pone.0074598.s014" target="_blank">Table S1</a>) between the interactions of poly(A)+ mRNA with β-tubulin (n=25, mean=2.3%, s.d.=1.5%), vimentin (n=33, mean=11.5%, s.d.=9.7%), or phalloidin (n=23, mean=53.7%, s.d.=19.9%). (<b>D</b>) The mean PLA frequency was significantly different (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074598#pone.0074598.s015" target="_blank">Table S2</a>) between the interactions of poly(A)+ mRNA with β-tubulin (n=25, mean=0.010µm^-3, s.d.=0.007µm^-3), vimentin (n=33, mean=0.019µm^-3, s.d.=0.016µm^-3), or phalloidin (n=23, mean=0.069µm^-3, s.d.=0.039µm^-3). Error bars, s.d.