Growth curve and immunohistologic characterization of BxPC-3 tumors grown on CAM.

<p>(A) Cells were implanted on CAM at embryonic day 11 and collected 2, 4, 5, 6 or 7 days after implantation. Macroscopic pictures were obtained at the same magnification from top, bottom and side view. Results are expressed as mean ± s.d., n>5 at each time-point. (B) Histologic (Haematoxylin-Eosin or Masson’s trichrome staining) analysis of tumors collected 2, 4, 5, 6 or 7 days after implantation. (C) Immunohistology of tumors 7 days after BxPC-3 implantation on CAM and human PDAC tumors. CK7 = Cytokeratin-7, CK19 = cytokeratin-19, CEA = Carcinoembryonic antigen, PAS = Amylase-periodic acid Schiff staining.