FIP1 expression decreases during adipogenesis, and by treatment with troglitazone.

<p><b>A</b>) Cell lysates were obtained from 3T3L1 fibroblasts (F) or fully differentiated adipocytes (A). Samples were separated by SDS-PAGE, transferred to a nitrocellulose filter and immunoblotted with an anti-FIP1 or β-tubulin antibody as shown. Representative blot of two independent experiments. <b>B</b>) Quantification of FIP1 protein levels (left panel) or FIP1 mRNA levels (right panel) following the differentiation of 3T3L1 cells to adipocytes. Data shows mean ± SEM of triplicate values. Statistic analysis: One way ANOVA * indicates p<0.05. <b>C</b>) 3T3L1 cells were cultured and differentiated to adipocytes. At day 8 of differentiation, cells were either left untreated or treated with vehicle or with 10 µM troglitazone for 3 days. Conditioned media and cellular lysates were harvested for adiponectin quantification by ELISA as described in the methods section A subset of cell plates were used for total RNA isolation and mRNA for FIP1 was quantified by real time PCR as described in the methods section. Data shows mean ± SEM of 2 independent experiments each performed in triplicate. Statistical analysis, one way ANOVA: * indicates p<0.05.