Examination of sRNA expression in <i>Y. pestis</i> WT, <i>hfq</i> and <i>crp</i> mutant strain.

<p>(A) Northern blot analysis of sRNA expression in <i>Y. pestis</i> WT (hfq⁺) and <i>hfq</i> mutant strain (hfq⁻). (B) Examination of sRNA expression in <i>Y. pestis</i> WT (crp⁺) and <i>crp</i> mutant strain (crp⁻). (C) Promoter analysis of Yp-sR084. TSS determination by primer extension assay is shown on the left panel. Lanes A, T, C and G represent the Sanger sequencing reactions. The transcription start sites were underlined. Organization and sequence features of sR084 are shown on the right panel. The start sites observed by primer extension are indicated by arrows (+1). Putative -10 and -35 regions of the promoters are boxed. The potential CRP binding sites that match CRP consensus [48] are indicated by dashed lines. The predicted <i>rho</i>-independent terminator is underlined.