Comparison of 144 APH-induced DNA breakpoints to predicted DNA topoisomerase I and II cleavage sites.

<p>(A) Topoisomerase I cleavage sites within <i>RET</i> intron 11 were predicted based on the consensus [5’-(A/T/G) (C/G/A) (A/T) (T/C)-3’] immediately upstream of the cleavage site [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075741#B51" target="_blank">51</a>], compared to APH-induced DNA breakpoints, and represented as the distance in bp from each APH breakpoint to the closest predicted cleavage site (x axis). A positive distance refers to the closest topoisomase I cleavage site being downstream of the APH breakpoint, and a negative distance being upstream. The percentage of all APH-induced breakpoints is displayed on the y axis. (B) Topoisomerase IIα cleavage sites were predicted using the consensus sequence [5’-(no A) (no T) (A/no C) (-) (C/no A) (-) (-) (-) (-) (no T) (-) (T/no G) (C/ no A) (-)-3’] [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075741#B52" target="_blank">52</a>], where breakage occurs between the nucleotides five and six. The locations of APH-induced breakpoints were compared to the predicted sites and represented in the same manner as in (A).