Correlation of staining patterns of all bands stained by all isotypes (A) or stained by IgTotal (B), IgM (C), and IgG (D) autoantibodies to CLL.

<p>Chickens were one year of age and divergently selected for 29 generations for High (red circles: Hg), Low (green diamonds: Lg), and Control (purple squares: Cg) agglutination titres to SRBC at 5 weeks of age, and C line birds phenotypically selected for high (yellow bars: Ch) or low (blue bars: Cl) NAb levels to KLH at 16 weeks of age. Ordination plots by PCA. Graphs include 11–12 birds from each line as also used for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072276#pone-0072276-g001" target="_blank">Figures 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072276#pone-0072276-g002" target="_blank">2</a>. Only CLL fragments that showed significant line differences for staining intensity (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072276#pone-0072276-t004" target="_blank">Tables 4</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072276#pone-0072276-t006" target="_blank">6</a>) are shown. ‘Eigenwaardes’ were 12.8% horizontal and 11.1% vertical axes (A), 17.9% horizontal and 11.6% vertical axes (B),16.5% horizontal and 15.4% vertical axis (C), and 20.8% horizontal and 12.5% verical axis (D), respectively. Numbers in <i>Ithalic</i> represent CLL fragments most informative for line clustering.