<p>(<b>A</b>) Luminescence imaging of <i>RD29A-LUC</i> expression in WT, <i>ros1</i> and <i>rdm16ros1</i>. (<b>B–C</b>) Real-time RT-PCR analysis of the expression of <i>RD29A-LUC</i> (<b>B</b>) and endogenous <i>RD29A</i> (<b>C</b>) in WT, <i>ros1</i> and <i>rdm16ros1</i> exposed to NaCl, abscisic acid (ABA) and cold (4°C) stresses. (<b>D</b>) Expression analysis of <i>ROS1</i> in WT, <i>ros1</i>, <i>rdm16ros1</i>, <i>nrpd1ros1</i> and <i>nrpe1ros1</i>.</p
<p>Methylation of the entire <i>ROS1</i> locus was examined in wild-type by bisulfite sequencing. Bi...
<p>(A) Relative luminescence intensity of WT, <i>rpt2a-2</i>, <i>met1-1</i> and <i>rpt2a-2met1-1</i>...
<p>(A) Heat map showing the accumulation of rdRNAs from genes involved in heme B biosynthesis or met...
<p>(<b>A</b>) All the morphological defects including dwarf stature, smaller silique and leaves in <...
<p>Top: Confirmation of <i>rdm1</i> transgene expression using RT-PCR. RNA from the corresponding ge...
<p>(<b>A–E</b>) Five Pol V-dependent loci showed decreased Pol V transcript levels in <i>rdm16ros1</...
RNA-directed DNA methylation (RdDM) is an important epigenetic mechanism for silencing transgenes an...
<p>(A) Relative luminescence intensity of RD29A::LUC in WT and <i>rpt2a-2</i>. Plants are untreated ...
<p>(A) The expression of stress responsive genes <i>RD29A</i>, <i>COR15A</i> and <i>COR47</i> in Col...
<p>(<b>A</b>) The inverted repeat construct used to express double stranded RNA matching the <i>ROS1...
<p>A. RDM1 is 163 amino acids in length. The only recognizable protein domain is DUF1950. The <i>rdm...
<p>(<b>A–C</b>) Morphological comparison of <i>rdm16ros1</i> with <i>ros1</i> with respect to plant ...
<p>(A) The effect of <i>mms19</i> on the silencing of <i>RD29A-LUC</i> and <i>35S-NPTII</i> transgen...
<p>(A) 35S::HPT in the Col-0 (WT) and <i>rpt2a-2</i> mutant on MS medium. Col-0 plants without any t...
<p>(<b>A</b>) Methylation of the 5' region of <i>ROS1</i> (region 1 in <a href="http://www.plosgenet...
<p>Methylation of the entire <i>ROS1</i> locus was examined in wild-type by bisulfite sequencing. Bi...
<p>(A) Relative luminescence intensity of WT, <i>rpt2a-2</i>, <i>met1-1</i> and <i>rpt2a-2met1-1</i>...
<p>(A) Heat map showing the accumulation of rdRNAs from genes involved in heme B biosynthesis or met...
<p>(<b>A</b>) All the morphological defects including dwarf stature, smaller silique and leaves in <...
<p>Top: Confirmation of <i>rdm1</i> transgene expression using RT-PCR. RNA from the corresponding ge...
<p>(<b>A–E</b>) Five Pol V-dependent loci showed decreased Pol V transcript levels in <i>rdm16ros1</...
RNA-directed DNA methylation (RdDM) is an important epigenetic mechanism for silencing transgenes an...
<p>(A) Relative luminescence intensity of RD29A::LUC in WT and <i>rpt2a-2</i>. Plants are untreated ...
<p>(A) The expression of stress responsive genes <i>RD29A</i>, <i>COR15A</i> and <i>COR47</i> in Col...
<p>(<b>A</b>) The inverted repeat construct used to express double stranded RNA matching the <i>ROS1...
<p>A. RDM1 is 163 amino acids in length. The only recognizable protein domain is DUF1950. The <i>rdm...
<p>(<b>A–C</b>) Morphological comparison of <i>rdm16ros1</i> with <i>ros1</i> with respect to plant ...
<p>(A) The effect of <i>mms19</i> on the silencing of <i>RD29A-LUC</i> and <i>35S-NPTII</i> transgen...
<p>(A) 35S::HPT in the Col-0 (WT) and <i>rpt2a-2</i> mutant on MS medium. Col-0 plants without any t...
<p>(<b>A</b>) Methylation of the 5' region of <i>ROS1</i> (region 1 in <a href="http://www.plosgenet...
<p>Methylation of the entire <i>ROS1</i> locus was examined in wild-type by bisulfite sequencing. Bi...
<p>(A) Relative luminescence intensity of WT, <i>rpt2a-2</i>, <i>met1-1</i> and <i>rpt2a-2met1-1</i>...
<p>(A) Heat map showing the accumulation of rdRNAs from genes involved in heme B biosynthesis or met...