Effect of TbKIN-C RNAi on TbKIN-D protein level and TbKIN-D RNAi on TbKIN-C protein level in the bloodstream form.

<p>(<b>A</b>). Western blot to detect TbKIN-D protein level in TbKIN-C RNAi cells and TbKIN-C protein level in TbKIN-D RNAi cells in the bloodstream form. (<b>B</b>). Western blot to detect TbKIN-D protein level in TbKIN-C RNAi cells and TbKIN-C protein level in TbKIN-D RNAi cells in the procyclic form. MG132 (25 µM) was added to TbKIN-C RNAi cells (day 2) and TbKIN-D RNAi cells (day 3) and incubated for 6 hrs. (<b>C</b>). TbKIN-C and TbKIN-D mRNA level in TbKIN-C RNAi cells and TbKIN-D RNAi cells measured by quantitative RT-PCR in the bloodstream form.