The genomic EMSA showed ORC-DNA selectivity.

<p>(A) Venn diagrams showing overlap between the complete set of annotated origins from oriDB or the complete set of yeast genes (annotated as of March 2011 on SGD) and the peaks called by ChIPotle (P value = 10⁻⁵) for the gEMSA data at 0.3, 3 and 30 nM ORC. (B) The fraction of base pairs in the yeast genome (y-axis) or in the gEMSA data set are shown for the genomic loci indicated on the x-axis. (C) Number of matches to the 11 bp ORC binding site consensus-motif (ACS) that were found in the indicated data sets. (D) The enrichment of an ACS motif (shown in (C)) was determined by plotting the normalized frequency (normalized to the frequency that the same motif is found within the whole genome) that an ACS match meeting a LOD cut-off of 70% was found within all of the base pairs comprising the relevant data set (x-axis). P-values for significance of enrichment indicated above bars.