IFN-α inhibition of DHBV cccDNA transcription requires protein synthesis.

<p>Dstet5 cells were treated and harvested as depicted in the top panel. DHBV mRNA (A) and cccDNA (B) were determined by Northern and Southern blot hybridization, respectively. Ribosomal RNAs served as loading controls for the Northern blot. The levels of Mx1 (C), OAS1 (D) and β-actin (E) mRNA were determined by semi-quantitative RT-PCR assay. pgRNA, pregenomic RNA; sRNA, mRNAs encoding envelope proteins; 28S and 18S, 28S and 18S rRNA, respectively; RC, relaxed circular DNA; DSL, double-stranded linear DNA.