Changes in mRNA expression levels in cells following differentiation.

<p>Undifferentiated and differentiated embryonic stem cells (ES), and 7 days and 10 days differentiated (7 d; 10 d) cells were analysed for the mRNA expression of stem cells (NANOG; OCT3/4), neuronal (NESTIN; MAP2) and dopaminergic markers (TH; Pitx3; c-RET; NURR1; LMX1B; ENG). (A) Equal amount of cDNA was amplified for each gene and representative image of agarose gel bands obtained following electrophoresis. (B) Band intensities as normalised by hypoxanthine-guanine phosphoribosyl transferase (HGPRT; as housekeeping gene), showing relative gene expression. Results are Mean ± S.E.M, n = 3–4. *<i>p</i>≤0.05 vs. ES group, #<i>p</i>≤0.05 vs. 7 d group; Student's t-test.